Product Description
The CleanPlex® Severe Combined Immunodeficiency (SCID) Panel is a pre-designed and made-to-order multiplex PCR / amplicon-based targeted sequencing (NGS) assay designed to examine the germline variants or mutations across 72 genes associated with Severe Combined Immunodeficiency (SCID). The panel targets all the exonic regions of those genes and the flanking intronic sequences. Compatible with just 10 ng of DNA, sequencing-ready libraries can be prepared using a streamlined workflow in just 3 hours. The pre-designed panel is optimized in silico to deliver data with high on-target performance and high coverage uniformity to ensure efficient use of sequencing reads.
This product is made to order. Once we receive your order, we will synthesize the panel and the kit will contain CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit. CleanPlex Indexed PCR Primers and CleanMag® Magnetic Beads can be ordered separately to complete the workflow from input DNA to sequencing-ready NGS libraries.
Storage Temperature
Store at -20 °C.
For Research Use Only. Not for use in diagnostic procedures.
Gene List:
ADA, AK2, ATM, BCL11B, BLM, CARD11, CD247, CD27, CD3D, CD3E, CD3G, CD40, CD40LG, CD8A, CIITA, CORO1A, DCLRE1C, DNMT3B, DOCK8, EPG5, FOXN1, IFNGR1, IKBKB, IL12RB1, IL2RA, IL2RG, IL7R, IRF8, ITGB2, ITK, JAK3, LAT, LCK, LIG4, LRBA, MAGT1, MALT1, MAP3K14, MSN, NHEJ1, ORAI1, PARN, PGM3, PIK3CD, PMS2, PNP, POLE, PRKDC, PTPRC, RAC2, RAG1, RAG2, RFX5, RFXANK, RFXAP, RMRP, RTEL1, SH2D1A, SMARCAL1, SP110, SPINK5, STAT1, STAT3, STAT5B, STIM1, STK4, TAP1, TAP2, TBX1, TYK2, WAS, ZAP70
References:
Dvorak CC, et al. The genetic landscape of severe combined immunodeficiency in the United States and Canada in the current era (2010-2018). JACI, 143(1):405 – 407.
Kung, C., Pingel, J., Heikinheimo, M. et al. Mutations in the tyrosine phosphatase CD45 gene in a child with severe combined immunodeficiency disease. 2000 Nat Med 6, 343–345